布魯斯·格林伍德 ... Batman / Bruce Wayne (voice)
詹森·阿克斯（Jensen Ackles）... Red Hood / Jason Todd (voice)
約翰·迪·瑪吉歐... Joker (voice) (as John DiMaggio)
尼爾·帕特裏克·哈裏斯 ... Nightwing / Dick Grayson (voice)
詹森·艾薩克... Ra's al Ghul (voice)
韋德·威廉斯... Black Mask (voice)
Carlos Alazraqui ... Chi Chi (voice)
Robert Clotworthy ... Leon (voice)
Gary Cole ... Bobo (voice)
Brian George ... Ra's Assistant (voice)
胡凱莉Kelly Hu ... Ms. Li (voice)
Phil LaMarr ... Rick (voice)
文森特·馬特拉 ... Robin / Jason Todd (voice)
Kevin Michael Richardson ... Tyler (voice)
Andrea Rotaobao... Reporter #1 / Additional Voices (voice)