奧德·菲爾 Oded Fehr ...... Carlos Olivera
艾麗·拉特 Ali Larter ...... Claire
伊恩·格雷 Iain Glen ...... Dr. Isaacs
亞香緹 Ashanti ...... Betty
克裏斯托弗·艾甘 Christopher Egan ...... Mikey
斯賓瑟·羅克 Spencer Locke ...... K-Mart
馬修·馬斯登 Matthew Marsden ...... Slater
林登·阿什比 Linden Ashby ...... Chase
Jason O'Mara ...... Albert Wesker
麥克·艾普斯 Mike Epps ...... L.J.
Joe Hursley ...... Otto
John Eric Bentley ...... Umbrella Tech
James Tumminia ...... Lab Tech
Kirk B.R. Woller ...... Scientist
Rick Cramer ...... Ice Hockey / Corridor Guard
Madeline Carroll ...... White Queen
Peter O'Meara ...... British Envoy
William Abadie ...... French Envoy
Ramón Franco ...... Runty
山恩·伍德森 Shane Woodson ...... Piggy
Valorie Hubbard ...... Ma
Geoff Meed ...... Pock Mark
Rusty Joiner ...... Eddie
Brian Steele ...... Rancid / Tyrant
Connor McCoy ...... Small Boy
Gary A. Hecker ...... Tyrant Vocal (voice)Gary Hecker)
Carolina Jimenez ...... Mirror Woman
Gary Hudson ...... Umbrella Captain (uncredited)